Stenekes, R.J.H. dextran Journal of Applied Polymer Science, 27, 795-810. Indo AM J Pharm. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). (2008). RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. Ubbelohde, Leo. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. ; Grishchenko, A.E. In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. 4K, b M.W. Structural and biocompatibility properties of dextran from Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. Epub 2022 Feb 2. Exp Cell Res. official website and that any information you provide is encrypted Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. Unauthorized use of these marks is strictly prohibited. Beer, M.U. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). Pharm Res. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. 1984;17(6):11703. European Polymer Journal, 37, 619-622. Eremeeva, T. E.; Bykova, T. O. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). Eng. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. (2003). The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. Analytical Chemistry. H&E stained images were analyzed using Image J software. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. 2010;4(1):117. The micropores of the human cadaver skin were regenerated according to the time. Dextran Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. 2009;6(4):34354. Correspondence to WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. Gekko, K. (1971). Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in Dilute solution properties and degree of chain branching For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. The Transdermal diffusion cell system was protected from light. The Hydrodynamic Radii of Macromolecules and Their Molecular weight, molecular weight distribution and molecular size of a native dextran. The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth We observe that this maximum radius is below The Viscosity of Dilute Solutions of Long-Chain Molecules. 2019;26(1):25261. Chemical penetration enhancers for transdermal drug delivery systems. Springer Nature. 1994, 95-0355. Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. ; Picout, D.R. Anal. Calculation of MarkHouwinkSakurada (MHS) equation viscometric constants for chitosan in any solventtemperatura system using experimental reported viscometric constants data. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. statement and Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Nanoparticles in magnetic resonance imaging: from simple to dual Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). Critical parameters for gel permeation The enhanced permeability through micropores was confirmed by Franz diffusion cell. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. eCollection 2015. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). 1905/05) for their financial support. Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). Table 2 compares the properties of nanoparticles coated with one or other polymer. Pharm Res. (2003). Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. "Dextrans in Aqueous Solution. The authors declare that they have no competing interests. Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. Unable to load your collection due to an error, Unable to load your delegates due to an error. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. 4K and M.W. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. De Belder, A.N. As a consequence of the diversity in their origin, the set of classical universal size independent quantities suffers from shortcomings. Kim J, Jang JH, Lee JH, Choi JK, Park WR, Bae IH, Bae J, Park JW. Macromolecules, 29, 2265-2268. Bahary, W.S. Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. Masuelli, Martin Alberto. International Journal of Biological Macromolecules, 23, 135-141. ; Wood, P.J. National Library of Medicine Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. Advanced Drug Delivery Reviews, 60, 1650-1662. @ Nilimesh See attached. (1987). IV. Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. Crini, G. (2005). The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. Front Microbiol. ; Rani, K.Y. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. Regulating dynamic signaling between hematopoietic stem cells In addition, we evaluated the regeneration of SC after microporation. Carbohydrate Research, 340, 811-826. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). GPC Symp. Carbohydrate Polymers 95 360-365. Radiofrequency with microneedling. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Dextran 670 GPC Standard | Pharmacosmos Dextran Pavlov, G.M. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. Dilute solution properties and degree of chain branching for dextran. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. 2009;301(8):60913. The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. Herwadkar A, Banga AK. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Cookies policy. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Carbohydrate Polymers, 68, 477-488. This conjecture found to be correct. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. 2013, Article ID 360239, pp. Federal government websites often end in .gov or .mil. MeSH terms Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). Carbohydrate Polymers, 39, 377-380. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. Biomater Res 24, 22 (2020). The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. Pharmacol Pharm. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. Dextran The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. Global hydrodynamic analysis of the molecular flexibility of galactomannans. Chen Rong Huei; Min Lang Tsai (1998). Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Reagenzien und Kits fr die Neurowissenschaft, Discover how to track and trace cells by imaging , Hier finden Sie unsere brigen Tracking-Produkte fr Sugetier-Zellkulturen, Molecular Probes Dextran-Konjugate Tabelle14.4. Ther. 4h after microporation, the sizes of the micropore decreased slightly. Webof overall macromolecular geometry and hydrodynamic draining. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. CAS Study of temperature influence. Gillespie, T.; Hulme, M. A. PMC Harding, Stephen E. (1997). Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. This situation is mainly the result of using dextans, solvent, additives, etc. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Macromolecules, 33, 5730-5739. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Colloid and Polymer Science, 286, 1223-1231. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. J. Braz. 2013;3(5):3588605. Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. WebR H Hydrodynamic radius [m] The basic DLS setup. To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. Viswanath, D.S. All authors have consented to the submission of this manuscript for publication. (1958). Molecular weight (M v) and hydrodynamic properties can be calculated from there. Figure4 shows the histological analysis of micropores in human cadaver skin. Rheology of dextran solutions. GYA primarily conducted research. Journal of Applied Polymer Science, 13, 2031-2032. Chem. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. Optical properties of dextran in solution and in films. Carbohydrate Polymers 87, 405-410. 2008;26(11):12618. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Dextrans can function efficiently as anterograde or retrograde tracers, Biomacromolecules 8, 2464-2475. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. hydrodynamic 7 8 Proc. Polysaccharides-based nanoparticles as drug delivery systems. Colloid Polym. Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Characterisation of insulin analogues therapeutically available to Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. J. Phys. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. Ed., 9(2), 85-90. American Journal of Food Science and Technology 2013, 1(3), 60-66. Drug Discov Today Technol. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. 2015;32(5):170413. The hydrodynamic radius of 2-NBDG glucose was too small to measure. Journal of Physical Chemistry. dextran The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. Masuelli, Martin Alberto (2013). Amersham Biosciences, AA Edition, www.amershambiosciences.com. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Carbohydrate Polymers, 36, 319-326. The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. (1999). Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). Ind. One day before the experiment, the skin was stabilized in the skin culture medium. Schematic illustration of transdermal delivery after the application of the microporation device. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). Progr. Recent Pat. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. Webwhittier union high school district superintendent. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. dextran hydrodynamic radius. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. Manage cookies/Do not sell my data we use in the preference centre. The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. J. Dextrans in Aqueous Solution. Experimental Review on Intrinsic The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. ; Aberle, T; Burchard, W. (2000). WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the 2018;1:10915. Before Int. Soc., 16, 3A, 426-433. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs.
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